Effect of tyrosine kinase inhibitors on CXCL12-induced chemotaxis of lymphocyte subsets.

(A) T cells either were left untreated or were pretreated with 10 to 1000 nM herbimycin A (HA), 10 to 1000 nM piceatannol (PC), or 10 to 1000 nM damnacanthal (DC) for 4 hours at 37°C. Chemotaxis assay was performed with 25 ng/mL CXCL12. Mean ± SD of 3 experiments (12 filters). *P < .005. **P < .001. ***P < .003. P values indicate comparison with cells not treated with inhibitors. (B) NK cells were either untreated or pretreated for 4 hours at 37°C with 1 to 100 nM HA, 1 to 100 nM PC, or 1 to 100 nM DC. Chemotaxis assay was performed with 10 ng/mL CXCL12. Mean ± SD of 3 experiments (18 filters). *P < .03. **P < .02. ***P < .07. P values indicate comparison with chemotaxis of cells not treated with inhibitors.