Effect of CXCL12 on tyrosine phosphorylation of Lck in lymphocytes.
CXCL12 induces tyrosine phosphorylation of Lck in lymphocytes. (A) (B) Lysates from T cells (4 × 107/mL) unstimulated (0) or stimulated with CXCL12 (200 ng/mL) for the indicated times, were immunoprecipitated (IP) with antiphosphotyrosine antibody (panel A) or anti-Lck (panel B) and immunoblotted with an antibody against phosphotyrosine (upper panels). The filters were stripped and reimmunoblotted with an antibody against Lck (lower panels). Representative for 4 independent experiments. (C) (D) Lysates from NK cells (4 × 107/mL) either unstimulated (0) or stimulated with CXCL12 (80 ng/mL) for the indicated times, were immunoprecipitated with either an antiphosphotyrosine antibody (panel C) or an anti-Lck antibody (panel D) and immunoblotted with an antibody against phosphotyrosine (upper panels). The filters were stripped and reimmunoblotted with an antibody against Lck (lower panels). Representative of 4 independent experiments.
