Effect of CXCL12 on kinase activity of Lck in lymphocytes.
CXCL12 increases kinase activity of Lck in lymphocytes. (A) T cells (4 × 107/mL) were stimulated with CXCL12 (200 ng/mL) for the indicated times. Lysates were subjected to Lck kinase activity assay. Mean ± SD of 3 experiments. (B) NK cells (4 × 107/mL) were stimulated with CXCL12 (80 ng/mL) for the indicated times. Lysates were subjected to Lck kinase activity assay. Mean ± SD of 3 experiments. (C) T cells (4 × 107/mL) were either untreated or pretreated with 1 μM HA, 1 μM PC, or 1 μM DC for 4 hours at 37°C. Cells were stimulated with 200 ng/mL CXCL12 for 1 minute, then subjected to the Lck kinase activity assay. Mean ± SD of 3 experiments. (D) NK cells (4 × 107/mL) were either untreated or pretreated with 100 nM PC or 100 nM DC for 4 hours at 37°C. Cells were stimulated with 80 ng/mL CXCL12 for 1 minute, then subjected to the Lck kinase activity assay. Mean ± SD of 3 experiments. *P < .05. **P < .03. ***P < .02. ****P < .01.
