Role of the Lck SH3 domain in mediating CXCL12-induced chemotaxis.

The Lck SH3 domain is important for mediating CXCL12-induced chemotaxis. (A) A schematic drawing of Lck demonstrating the 3 different Lck constructs used and the position of the point mutations. (B) An immunoblot with an antibody against Lck, demonstrating successful transfection of Lck in the 3 different transfectants. (C) Analysis of surface expression of CXCR4 in untransfected cells and in the 3 different transfectants. (D) The 3 different constructs or an empty vector were transiently transfected into the JCaM1.6 cell line and subjected to a chemotaxis assay toward CXCL12 (25 ng/mL). Mean ± SD of 3 experiments (18 filters). *P < .004 as compared with Lck wt–transfected cells.