Glycosylation of yeast-expressed Epo (R103A).

Epo (R103A) was digested with N-glycanase to determine the extent of N-linked glycosylation. N-glycanase treatment is indicated with + and no treatment is indicated with −. The left gel shows glycosylation detected using glycan stain. The open arrow indicates residual glycan staining due to O-linked glycosylation. The right gel shows a Western blot using anti-Epo antibody. The blot was overloaded to demonstrate complete digestion.