Fig. 3.
Epo (R103A) inhibits Epo-dependent growth of 32D/EpoR cells.
32D/EpoR WT cells (A) or 32D/EpoR FE cells (B) were grown were grown for 4 days in Epo (15.5 ng/mL, solid circle) or IL-3 (2 ng/mL, open square) in the presence of the indicated concentration of Epo (R103A). Initial cell number was 5 × 103 cells/well in 150 μL culture media. Cell viability after 4 days of growth was determined using MTS reagent. Each point was done in quadruplicate with SE bars shown.
