Defect in Slug mutant mice is intrinsic to the stem cell.

(A) Kit immunoprecipitations from BM-derived mast cells were probed for kit and reprobed for phosphotyrosine. Slug+/−, heterozygous mice; Slug−/−, homozygous mice; Slug+/+, wild-type mice. (B, C) Analysis of the hematopoietic system in normal recipient mice reconstituted with either Slug+/+ HSC or Slug−/− HSC by FACS. BM, spleen, and thymus samples were stained with monoclonal antibodies and were analyzed by flow cytometry. The hematopoietic composition of mice reconstituted with Slug−/− HSC is similar to that for Slug−/−mice. A representative analysis of the c-kit⁺ cells present in the BM and spleen of normal recipient mice reconstituted with either Slug+/+ HSC or Slug−/− HSC after the induction of hemolytic anemia with PHZ is shown.