KGF and FgfR2-IIIb are expressed intrathymically.

CD45⁺ thymocytes (T) and CD45⁻ MHC class II⁺ stromal cells (TECs) were FACS isolated. The CD45⁻ MHC class II⁺ cells included TECs and fibroblasts. Expression of the TEC-derived cytokines, IL-7 and SCF, and FgfR2-IIIb and KGF by intrathymic subpopulations was analyzed by RT-PCR. β-actin was used as a control for loading of RNA.