Effect of oxLDL on the procoagulant activity of human aortic SMCs, macrophages, and aortic ECs.
SMCs (A), macrophages (B), or HAECs (C) in the amount of 2 × 105/well were incubated in the absence of lipoproteins (○) or in the presence of 100 μg/ml oxLDL (●) or native LDL (▴) for 12 hours at 37°C. Following incubation, the conversion of fX (170 nM) into fXa catalyzed by fIXa (2 nM) and thrombin-activated fVIII (1 nM) was measured in a chromogenic assay as described in “Materials and methods.” In the control experiment (⋄), the Xase reaction was performed in wells incubated with oxLDL in the absence of cells. In the additional control experiment (+), the Xase reaction was performed using fVIII activated by thrombin, which was subsequently inactivated by excess of hirudin. Each data point represents the mean value ± SD of triplicates.
