Effect of oxLDL on translocation of PS to the outer leaflet of cell membranes.

Human SMCs (A,B), macrophages (C,D), and HAECs (E,F) were grown in Lab-Tek II chamber slides and incubated in the absence (A,C,E) or presence (B,D,F) of 100 μg/mL oxLDL for 12 hours. Exposure of PS on cell membranes was tested by immunofluorescence using annexin V coupled with EGFP as described in “Materials and methods.” Microscopy was performed using an Eclipse E800 microscope (Nikon) equipped with fluorescent filter blocks at magnification × 600.