Patterns of localization of fVIII and oxLDL in atherosclerotic lesions.

Human coronary atherectomy sections containing normal media (A,C,E) and atheromatous regions (B,D,F) were stained for oxLDL (A,B) or fVIII (C,D) using mAbs OXL41.1 and ESH8, respectively, and double stained for both components (E,F). The single and double staining were performed as described in “Materials and methods.” Positive staining for oxLDL and fVIII in panels B and D is brown; cell nuclei in panels A through D are counterstained with hematoxylin. In double-stained images, panels E and F, accumulations of fVIII (blue) and oxLDL (brown) frequently overlap as evidenced by presence of areas stained black. Cell nuclei in panels E and F were counterstained with nuclear fast red. Microscopy was performed using an Eclipse E800 microscope at magnification × 600.