Fig. 7.
Fig. 7. Effect of oxLDL on thrombin generation via the intrinsic pathway. / Human SMCs (A), macrophages (B), or HAECs (C) in the amount of 2 × 105/well were incubated in the absence of lipoproteins (○) or in the presence of 100 μg/mL oxLDL (●) or native LDL (▴) for 12 hours at 37°C. Following incubation, the conversion of prothrombin (1.4 μM) into thrombin in the presence of fVIII (1 nM), fIXa (2 nM), fV (20 nM), and fX (170 nM) was measured in a chromogenic assay as described in “Materials and methods.” In the control experiment (⋄), the reaction was performed in wells incubated with oxLDL in the absence of cells. Each data point represents the mean value ± SD of triplicates.

Effect of oxLDL on thrombin generation via the intrinsic pathway.

Human SMCs (A), macrophages (B), or HAECs (C) in the amount of 2 × 105/well were incubated in the absence of lipoproteins (○) or in the presence of 100 μg/mL oxLDL (●) or native LDL (▴) for 12 hours at 37°C. Following incubation, the conversion of prothrombin (1.4 μM) into thrombin in the presence of fVIII (1 nM), fIXa (2 nM), fV (20 nM), and fX (170 nM) was measured in a chromogenic assay as described in “Materials and methods.” In the control experiment (⋄), the reaction was performed in wells incubated with oxLDL in the absence of cells. Each data point represents the mean value ± SD of triplicates.

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