Effect of oxLDL on the prothrombinase activity.

Human SMCs (A) or macrophages (B) were incubated in the absence (○) or in the presence (●) of oxLDL as described in Figure 7. Following incubation, the conversion of prothrombin (1.4 μM) into thrombin in the presence of fXa (8 nM) and fV (20 nM) was measured in a chromogenic assay as described in “Materials and methods.” In the control experiment (⋄), the reaction was performed in wells incubated with oxLDL in the absence of the cells. Each data point represents the mean value ± SD of triplicates.