Fig. 1.
Fig. 1. Characterization of VEGF-D. / (A) VEGF-DΔNΔC/FLAG (20 μg purified protein) was analyzed by SDS-PAGE under reducing conditions and was detected by silver staining (lane 1) or Western blotting with anti-FLAG2 or anti–VEGF-D3 antibodies. (B) Thymidine incorporation into endothelial cells induced by VEGF-DΔNΔC/FLAG. (C) Effect of VEGF-DΔNΔC/FLAG on endothelial cell migration toward vitronectin (10 μg/mL) in a modified Boyden chamber. (D) Specificity of endothelial cell migration induced by VEGF-DΔNΔC/FLAG. Effects of VEGFR-2-Fc chimera and anti–VEGFR-2 or anti-αVβ3 blocking antibodies (10 μg/mL each) are shown. Data are means ± SD of 3 separate experiments.

Characterization of VEGF-D.

(A) VEGF-DΔNΔC/FLAG (20 μg purified protein) was analyzed by SDS-PAGE under reducing conditions and was detected by silver staining (lane 1) or Western blotting with anti-FLAG2 or anti–VEGF-D3 antibodies. (B) Thymidine incorporation into endothelial cells induced by VEGF-DΔNΔC/FLAG. (C) Effect of VEGF-DΔNΔC/FLAG on endothelial cell migration toward vitronectin (10 μg/mL) in a modified Boyden chamber. (D) Specificity of endothelial cell migration induced by VEGF-DΔNΔC/FLAG. Effects of VEGFR-2-Fc chimera and anti–VEGFR-2 or anti-αVβ3 blocking antibodies (10 μg/mL each) are shown. Data are means ± SD of 3 separate experiments.

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