Induction of myeloid gene expression following granulocytic differentiation in C/EBPα-expressing and nonexpressing cells.

(A) The 10α^−/−αER cells were differentiated following induction of C/EBPα activity by treatment with β-estradiol for the number of days as indicated above each figure. The Northern blot demonstrates induction of MPO and IL-6Rα. The blot was hybridized to GAPDH (bottom panel) as a control for RNA loading and integrity. (B) The 13α^−/− cells were induced with IL-3 and GM-CSF for the number of days as indicated. The Northern blot demonstrates induction of MPO RNA. The blot was hybridized with GAPDH as a loading control as indicated.