Induction of C/EBPβ and C/EBPε protein is associated with induction of C/EBPα-independent granulocytic differentiation but not with ATRA treatment.

C/EBP proteins were detected by Western blot analysis. A monoclonal antibody recognizing β-tubulin was used to control for levels of protein in each lane. Indicated above each blot are the days following induction of granulocytic differentiation with (A) ATRA and (B) IL-3 and GM-CSF. The blots in panel A were exposed longer than those in panel B to visualize C/EBPβ and C/EBPε proteins.