Up-regulation of C/EBPβ and C/EBPε protein is also associated with C/EBPα-induced granulopoiesis.

C/EBP proteins were detected by Western blot, and β-tubulin was used as a loading control. (A) Whole cell lysates were harvested before and 2, 4, and 6 days after induction of exogenous C/EBPα protein after β-estradiol treatment in 10α^−/−αER cells. (B) Whole cell lysates from line 13α^−/− before and after β-estradiol and G-CSF treatment. Days of treatment (2, 4, 6, and 8 days) are indicated. The blots in panel B were exposed longer than those in panel A to visualize C/EBPβ and C/EBPε proteins.