Fig. 2.
Fig. 2. TEL-JAK2 expression results in constitutive tyrosine phosphorylation of Ship-1 and association with the SH2 domain of Ship-1. / (A) Ba/F3 and Ba/F3-TEL-JAK2 cells lines were depleted of cytokine and then stimulated in the presence (+) or absence (−) of IL-3. IPs were performed using a Ship-1 antibody, and tyrosine-phosphorylated proteins were detected by pTyr IB (upper panel). Total Ship-1 was detected by reprobing with a Ship-1 antibody (lower panel). (B) Ba/F3 and Ba/F3-TEL-JAK2(5-19) cells were depleted of cytokine and then stimulated in the presence (+) or absence (−) of IL-3. GST in vitro mixes were performed using 5 μg GST (lanes 1-4), GST–Ship-1 SH2 domain (lanes 5-8), or GST–Ship-1 Arg34Gln (lanes 9-12). Ba/F3 lysates are shown (lanes 13, 14). Tyrosine-phosphorylated proteins were detected by IB with a pTyr antibody.

TEL-JAK2 expression results in constitutive tyrosine phosphorylation of Ship-1 and association with the SH2 domain of Ship-1.

(A) Ba/F3 and Ba/F3-TEL-JAK2 cells lines were depleted of cytokine and then stimulated in the presence (+) or absence (−) of IL-3. IPs were performed using a Ship-1 antibody, and tyrosine-phosphorylated proteins were detected by pTyr IB (upper panel). Total Ship-1 was detected by reprobing with a Ship-1 antibody (lower panel). (B) Ba/F3 and Ba/F3-TEL-JAK2(5-19) cells were depleted of cytokine and then stimulated in the presence (+) or absence (−) of IL-3. GST in vitro mixes were performed using 5 μg GST (lanes 1-4), GST–Ship-1 SH2 domain (lanes 5-8), or GST–Ship-1 Arg34Gln (lanes 9-12). Ba/F3 lysates are shown (lanes 13, 14). Tyrosine-phosphorylated proteins were detected by IB with a pTyr antibody.

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