Fig. 4.
Fig. 4. Grb2 constitutively associates with TEL-JAK2 fusions through the SH2 domain of Grb2. / (A) Ba/F3 and Ba/F3-TEL-JAK2 cell lines were depleted of cytokine and then stimulated in the presence (+) or absence (−) of IL-3. IPs were performed using a Grb2 antibody, and tyrosine-phosphorylated proteins were detected by pTyr IB (upper panel). Total Ship-1, Shc, and Grb2 proteins were detected upon reprobing with Ship-1, Shc, and Grb2 antibodies, respectively (lower panels). (B) Ba/F3 and Ba/F3-TEL-JAK2 cell lines were depleted of cytokine and stimulated in the presence (+) or absence (−) of IL-3. GST in vitro mixes were performed using 5 μg GST (lanes 1-8) or GST-Grb2 SH2 domain (lanes 9-16). Tyrosine-phosphorylated proteins were detected by IB with a pTyr antibody.

Grb2 constitutively associates with TEL-JAK2 fusions through the SH2 domain of Grb2.

(A) Ba/F3 and Ba/F3-TEL-JAK2 cell lines were depleted of cytokine and then stimulated in the presence (+) or absence (−) of IL-3. IPs were performed using a Grb2 antibody, and tyrosine-phosphorylated proteins were detected by pTyr IB (upper panel). Total Ship-1, Shc, and Grb2 proteins were detected upon reprobing with Ship-1, Shc, and Grb2 antibodies, respectively (lower panels). (B) Ba/F3 and Ba/F3-TEL-JAK2 cell lines were depleted of cytokine and stimulated in the presence (+) or absence (−) of IL-3. GST in vitro mixes were performed using 5 μg GST (lanes 1-8) or GST-Grb2 SH2 domain (lanes 9-16). Tyrosine-phosphorylated proteins were detected by IB with a pTyr antibody.

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