Fig. 8.
Fig. 8. Expression of TEL-JAK2(5-19)Tyr314Phe in Ba/F3 cells does not affect factor-independent growth. / (A) Ba/F3 cells expressing the indicated TEL-JAK2 constructs were depleted of cytokine and then stimulated in the presence (+) or absence (−) of 10 ng/mL IL-3. IPs were performed with a pan-Jak antibody, and tyrosine-phosphorylated proteins were detected by IB with pTyr antibody. (B) A total of 2000 Ba/F3 cells (closed squares) and Ba/F3 cells expressing TEL-JAK2(4-17) (open diamonds), TEL-JAK2(5-19) (open circles), and TEL-JAK2(5-19)Tyr314Phe (open triangles) constructs were seeded into 96-well plates containing media of increasing IL-3 concentration. The cells were incubated for 48 hours at 37°C prior to the addition of XTT and PMS. Absorbance was read at 450 nm following 4 hours of incubation at 37°C.

Expression of TEL-JAK2(5-19)Tyr314Phe in Ba/F3 cells does not affect factor-independent growth.

(A) Ba/F3 cells expressing the indicated TEL-JAK2 constructs were depleted of cytokine and then stimulated in the presence (+) or absence (−) of 10 ng/mL IL-3. IPs were performed with a pan-Jak antibody, and tyrosine-phosphorylated proteins were detected by IB with pTyr antibody. (B) A total of 2000 Ba/F3 cells (closed squares) and Ba/F3 cells expressing TEL-JAK2(4-17) (open diamonds), TEL-JAK2(5-19) (open circles), and TEL-JAK2(5-19)Tyr314Phe (open triangles) constructs were seeded into 96-well plates containing media of increasing IL-3 concentration. The cells were incubated for 48 hours at 37°C prior to the addition of XTT and PMS. Absorbance was read at 450 nm following 4 hours of incubation at 37°C.

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