Effect of oxidized EPA on neutrophil and monocyte adhesion to endothelial cells.

HUVECs were pretreated for 1 hour with vehicle alone (Veh, PBS plus oxidizing reagents) or native (EPA) or oxidized EPA (oxEPA) at the indicated concentrations. HUVECs were then stimulated with LPS for 5 hours and assayed for human neutrophil (A) or monocyte (B) adhesion. (C) Endothelial cells were pretreated for 1 hour with vehicle alone (Veh), 100 μM native (EPA), or oxidized EPA (oxEPA). HUVECs were then incubated with TNF-α (stippled bars) or IL-1β (solid bars) and assayed for neutrophil adhesion. Results are expressed as number of leukocytes attached per well of endothelial cells (± SEM). *P < .00001 compared with EPA + LPS or IL-1β or TNFα, and Veh + LPS or IL-1 or TNF, n = 3.