Endothelial VWF recruits platelets to the vessel wall.

(A) In the ex vivo flow chamber inhibition of the VWF-A1 domain by the blocking mAb AJvW-2 reduced translocation and firm adhesion almost to control levels (*P < .01). (B) In autoradiographs for VWF expression with the iodine-labeled antibody AJvW-2, control arteries (n = 2) did not show localized enhancement of radioactive signal. Aortas from rabbits on a diet for 3 months (n = 6, right panel) expressed VWF primarily at segmental artery ostia, indicating that hyperlipidemia induces endothelial VWF expression before lesions develop.