SCF-induced protection from cell death in G1E-ER2 cells cocultured on stroma expressing different isoforms of SCF.

G1E-ER2 cells were cocultured for 48 hours with parental Sl/Sl4 stromal cells or stable stromal cell transfectants expressing S-SCF, MA-SCF, or MR-SCF. Total cell death was quantitated by performing annexin V and propidium iodide (PI) staining as described in “Materials and methods.” Bars denote the percentage of total cell death (± SEM) of 2 independent experiments performed in replicates of 3. *P < .05 Sl/Sl4 versus S-SCF, MA-SCF, and MR-SCF. **P < .05 MR-SCF versus MA-SCF, S-SCF, and Sl/Sl.4