IMiD3 enhances MM cell death induced by TRAIL/Apo2L and PS-341.

(A) Combined proapoptotic effect of IMiD3 with TRAIL/Apo2L in MM.1S cells. Cells were pretreated with or without 1 μM IMiD3 for 4 hours, and a low concentration of TRAIL/Apo2L (50 ng/mL) was then added for an additional 24 hours. IMiD3 potentiated the apoptotic effect of TRAIL/Apo2L. Data shown (mean ± SD) are representative of 3 experiments. (B) Combined proapoptotic effect of IMiD3 with PS-341 in MM.1S cells. Cells were pretreated with or without 1 μM IMiD3 for 48 hours, and then a subtoxic concentration of PS-341 (3 nM) was added for an additional 24 hours. IMiD3 potentiated the apoptotic effect of PS-341. Data shown (mean ± SD) are representative of 3 experiments.