Intracellular expression of p55 (IL-2Rα) and p75 (IL-2Rβ) proteins.
Cells were treated with 10−6 M or 10−8M alitretinoin (Panretin) or bexarotene for 48 hours. The expression of p55 and p75 was analyzed by immunoblot. (A) Lane 1, untreated HUT78 cells (control); lane 2, 10−10 M rexinoid-treated cells; lane 3, 10−8 M rexinoid-treated cells. (B) Lane 1, untreated fresh Sezary cells; lanes 2-5, Sezary cells after exposure to 10−10 M Panretin (lane 2), 10−8 M Panretin (lane 3), 10−10 M bexarotene (lane 4), 10−8 M bexarotene (lane 5). (C) Expression of p75 in NALM6 (lanes 1-6) and CLL cells (lanes 7-11) after rexinoids; lanes 1, 4, and 7, untreated; lanes 2 and 8, 10−10 M Panretin; lanes 3 and 9, 10−8 M Panretin; lanes 5 and 10, 10−8 M bexarotene; lanes 6 and 11, 10−10 M bexarotene. The “a” represents protein band and the “b” represents the constitutively expressed on Coomassie blue–stained protein bands as a control for protein loading.
