Western blot and flow cytometric analysis of SM-LCL.

(A) Western blot analysis of SM-LCL. Cell lysates of 1B1-LCL (healthy control) and SM-LCL were put into reactions with antibodies as indicated. Tpn indicates tapasin; Tpn-Nter, tapasin molecules that were detected by the anti–N-terminal tapasin antibody, Rgp48N. In the Tpn-Nter lane, portions around 48 kd (normal tapasin) and 18 kd (putative truncated tapasin) were shown in 1B1-LCL and SM-LCL, respectively. (B) Flow cytometric analysis. Cell surface HLA class I expression was detected by monoclonal antibody FITC-w6/32. Cells were SM-LCL (Tpn-deficient), KMW-B2 (TAP1-deficient), and 1B1-LCL (healthy control). Thin dotted line indicates control FITC–immunoglobulin G.