Fig. 3.
Fig. 3. Incubation of SMCs with cycloheximide (CX) and actinomycin D. / The effects of CX and ActD on HO-1 mRNA (A) and protein (B) expression by serum in vascular SMCs are shown. Cells were treated with serum (10%) in the presence or absence of CX (5 μg/mL) or actinomycin D (ActD) (2 μg/mL) for 8 hours and then analyzed for HO-1 mRNA and protein. Similar findings were observed in 3 separate experiments.

Incubation of SMCs with cycloheximide (CX) and actinomycin D.

The effects of CX and ActD on HO-1 mRNA (A) and protein (B) expression by serum in vascular SMCs are shown. Cells were treated with serum (10%) in the presence or absence of CX (5 μg/mL) or actinomycin D (ActD) (2 μg/mL) for 8 hours and then analyzed for HO-1 mRNA and protein. Similar findings were observed in 3 separate experiments.

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