Effect of CO on retinoblastoma phosphorylation in vascular SMCs.

Cells were treated with serum (0%-10%) and SnPP (20 μM) for 24 hours in the presence and absence of CO (100 ppm) and retinoblastoma expression determined using an antibody that recognizes both the hypophosphorylated (pRb) and hyperphosphorylated (pRb-P) forms of pRb (A) or a phosphorylation-specific pRb antibody (B). Similar findings were observed in 4 separate experiments.