Solid-phase binding and kinetics of PC hydrolysis.

(A) Solid-phase binding of WT (●) and Arg67His mutant (▵) thrombin to recombinant immobilized TM. The experimental conditions are reported in the text. Continuous lines were drawn according to the best-fit K_d values listed in Table 1, with vertical bars expressing the SE. (B) Kinetics of PC hydrolysis by WT (●) and Arg67His mutant (▵) thrombin, under pseudo–first-order conditions. Continuous lines were drawn with the best-fit parameter values of k_cat/K_m reported in Table 1. The SEs are expressed by the vertical bars.