Effect of long-term exposure to G-CSF on peripheral blood cell counts and BM colony formation.

(A) RBC and (B) WBC counts in Fancc^−/− mice before and after one MMC injection prior to cytokine therapy (■, no cytokines; ⋄, G-CSF; ●, EPO; ▪, EPO + G-CSF). Mice were subjected to a MMC challenge after 18 weeks of cytokine treatment, and blood cell counts were monitored for the following 8 weeks, at which point the experiment was terminated and BFU-E (C) and CFU-GM (D) were analyzed. (E) Survival curves of Fancc^−/− mice before (after 18 weeks of cytokine treatment) and following MMC challenge (■, no cytokines; ⋄, G-CSF; ●, EPO; ▪, EPO + G-CSF). Each point represents the mean ± SEM of 2 to 6 mice. The absence of SEM bars indicates that values were too low to appear in the graph. Significant differences compared to untreated controls: *,P < .05 evaluated by 1-way ANOVA.