Fig. 4.
Expansion of nonadherent VEGFR-3+ cells in liquid culture.
A quantity of 1 × 105 freshly isolated CD34+cells from human fetal liver were incubated for 2 weeks in EC growth medium. (A) The total number of viable nonadherent cells (○) in the cultures was determined at 1, 3, 5, 7, and 14 days. (B) The number of nonadherent VEGFR-3+ cells (▴) in the cultures was determined at 1 and 14 days by flow cytometry. The results are given as means ± SEs of 3 independent experiments. During the 2-week culture, a strong proliferation (more than 40-fold) of nonadherent VEGFR-3+ cells can be seen.
