Flow cytometric analysis of surface marker expression on VEGFR-3⁺ cells in isolated CD34⁺ cells, in monolayers generated from isolated CD34⁺ cells, and in HUVEC monolayers.

Isolated cells coexpress VEGFR-3⁺ and stem/progenitor cell markers CD133 and CD34 (A). All adherent VEGFR-3⁺ cells in monolayers derived from isolated CD34⁺ cells incorporate Ac-LDL, confirming that they are ECs (B). Adherent VEGFR-3⁺cells expressing endothelial markers CD105 (C) or CD51/61 (D) are also detected. VEGFR-3⁺ cells found in HUVEC monolayers have a surface marker expression pattern similar to that of adherent VEGFR-3⁺ ECs derived from CD34⁺ cells (panel A).