Fig. 2.
![Fig. 2. Effect of SCF on DNA fragmentation of irradiated Ba/F3-Kit cells. / Cells were labeled with 0.5 μCi/mL [3H]thymidine (1.8 × 104 Bq/mL) for 48 hours. Cells were then washed, treated (○) or not (●) with SCF (200 ng/mL for 30 minutes), and irradiated at 4 Gy. At indicated times, cells were embedded in agarose plugs and digested and the DNA was separated by high-voltage electrophoresis. Fragmented and unfragmented DNA-associated radioactivities were quantitated. The results are expressed as percentage of fragmented over total DNA-associated radioactivity. Results are the mean ± SD of 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/4/10.1182_blood.v100.4.1294.h81602001294_1294_1301/6/h81622982002.jpeg?Expires=1735541332&Signature=JUDlh~pVkWqWEj4xVnfVGxVZh6HqW5MDTe2-wX6bddVGQbtcKwXS8bkMe~nhhoDor3nLYnCiBxRnu25AfIRAm47TUj4jORvQ~LGkbBSWj0MlJQAPF2AKlMuvsmTyRVttnsuMnYAopcg9PDROHsATk4Q3DHklnvBjZ1GZMmivJ852~Us8~N~ZBNTW7wnbCrf~wJ3SaZtFaYvEVpfS3hlFh2ljZ9T1NjTUMmrqo7BgEWBPDSI~jJoWFeg2MeUSLqQXmt4GxS6e2~Cd8RcqV5NKQNDjO5KgGx~rEgdcWTpEO4IsSBP9pefo~WwWKuAA2MZJFub3jELcYso2sioov27NUA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of SCF on DNA fragmentation of irradiated Ba/F3-Kit cells.
Cells were labeled with 0.5 μCi/mL [3H]thymidine (1.8 × 104 Bq/mL) for 48 hours. Cells were then washed, treated (○) or not (●) with SCF (200 ng/mL for 30 minutes), and irradiated at 4 Gy. At indicated times, cells were embedded in agarose plugs and digested and the DNA was separated by high-voltage electrophoresis. Fragmented and unfragmented DNA-associated radioactivities were quantitated. The results are expressed as percentage of fragmented over total DNA-associated radioactivity. Results are the mean ± SD of 3 independent experiments.
