Fig. 3.
![Fig. 3. Effect of SCF on ceramide generation in irradiated Ba/F3-Kit cells. / Cells were prelabeled with [3H]palmitate for 48 hours. Cells were then washed, treated (○) or not (●) with SCF (200 ng/mL for 30 minutes), and irradiated at 4 Gy. Following incubation during the indicated times, aliquots (3 × 106 cells/mL) were collected and lipids were extracted. Labeled ceramide was resolved by thin-layer chromatography and identified as described in “Materials and methods.” Results are the mean ± SD of 3 independent experiments (*P < .05).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/4/10.1182_blood.v100.4.1294.h81602001294_1294_1301/6/h81622982003.jpeg?Expires=1735541185&Signature=Tho4gJm5r9YlpC9oOzcmNUVBWUMvEp5j5P72STcSHcKEwO2mrzqSdWAO6DlA8lOd2Qs6U8cYeMVxuJjgKYedbGDVMU~f4QizZt3-84ZcKrIMzc-MX-CK1j9OHNPTBvsKIvLhubs449l7RVRFePs~woLssoGJIlUQvMkR48AF5eXcTQmlRrJSwhagwLIg7cd4Nrm2~TgdrVFZdp5iNKDyltu9uHd7-etTyOykUH~qmW8lRNiXIQfcuJJw~sdYgEam3-slRv7LjxAbC-h--wlC-D1PrFUmDbklrMSNdcXtS1i2Mk42v8nbE6vE6OCFWVP4V57bLausbpSFcbzSA3Nv7Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of SCF on ceramide generation in irradiated Ba/F3-Kit cells.
Cells were prelabeled with [3H]palmitate for 48 hours. Cells were then washed, treated (○) or not (●) with SCF (200 ng/mL for 30 minutes), and irradiated at 4 Gy. Following incubation during the indicated times, aliquots (3 × 106 cells/mL) were collected and lipids were extracted. Labeled ceramide was resolved by thin-layer chromatography and identified as described in “Materials and methods.” Results are the mean ± SD of 3 independent experiments (*P < .05).
