Influence of SCF stimulation on ionizing radiation-induced N-SMase stimulation and ceramide generation in 32D-Kit cells.

Cells were pretreated or not with SCF (200 ng/mL for 30 minutes) and irradiated with 4 Gy. N-SMase activity and ceramide levels were performed as described previously. Results are shown at maximum ceramide production (A) and N-SMase stimulation (B) (10-15 minutes) and are the mean ± SD of 3 independent experiments (*P < .05).