Influence of SCF stimulation on ionizing radiation-induced N-SMase stimulation and ceramide generation in 32D-KitYF719 cells.

Cells were pretreated or not with SCF (200 ng/mL for 30 minutes) and irradiated at 4 Gy. N-SMase activity and ceramide levels were performed as previously described. Results are shown at maximum ceramide production (A) and N-SMase stimulation (B; 10-15 minutes) and are the mean ± SD of 3 independent experiments (*P < .05).