Influence of SCF stimulation on irradiated-induced N-SMase stimulation and ceramide generation in 32D-KitYF728 cells.

32D-KitYF728 cells were pretreated or not with SCF (200 ng/mL for 30 minutes) and irradiated at 4 Gy. N-SMase activity and ceramide production were measured as previously described. Results are shown at maximum ceramide production (A), N-SMase stimulation (B; 10-15 minutes). (C) Apoptosis evaluated by DAPI staining at 24 hours of 32D-Kit (░,) and 32D-KitYF728 cells (▪, ■) irradiated at 4 Gy. Values are the mean ± standard deviation of 3 independent experiments (*P < .05).