Inhibition of cytoadherence on resting and stimulated HDMECs by PpMC-179 in vitro.

Resting and TNF-α–stimulated (10 ng/mL, 24 hours) endothelial monolayers were preincubated with 2 μM PpMC-179 (30 minutes at 37°C). IRBCs were then perfused over the monolayers at 1 dyne/cm². In some experiments, monolayers were preincubated with PpMC-179 or both PpMC-179 and the inhibitory anti–ICAM-1 mAb 84H10 at 5 μg/mL. (A,C). Adhesion of IRBCs on unstimulated (n = 4) and TNF-α–stimulated HDMECs (n = 5) was significantly reduced by PpMC-179, and the combination of PpMC-179 and anti–ICAM-1 (n = 2). *P < .05 and **P < 0.01 compared to control. (B,D). Rolling flux on resting or stimulated HDMECs was unaffected by PpMC-179, but was inhibited by the combination of PpMC-179 and an anti–ICAM-1 mAb. *P < .05 compared to control.