Fig. 1.
Fig. 1. Increased frequency and replating potential of HPP-CFCs from Smad5−/− yolk sacs at E9.0 to 9.5. / Dispersed yolk sac cells were cultured in HPP-CFC medium and the number of macroscopic colonies was counted after 14 days of culture (A). Then individual plucked macroscopic colonies were resuspended and plated into secondary replating system and the number of secondary colonies was scored after 7 days of culture (B). Results of HPP-CFC assay were from 5 wild-type, 8 Smad5+/−, and 6 Smad5−/−embryos and are expressed as means ± SEMs. Results of replating assay were obtained from 36 wild-type, 36 heterozygous, and 72 Smad5−/− HPP-CFC macroscopic colonies of 3 yolk sac cultures, respectively, and are expressed as means ± SEMs. *represents data found to be significantly different from the corresponding control values (P < .05).

Increased frequency and replating potential of HPP-CFCs from Smad5−/− yolk sacs at E9.0 to 9.5.

Dispersed yolk sac cells were cultured in HPP-CFC medium and the number of macroscopic colonies was counted after 14 days of culture (A). Then individual plucked macroscopic colonies were resuspended and plated into secondary replating system and the number of secondary colonies was scored after 7 days of culture (B). Results of HPP-CFC assay were from 5 wild-type, 8 Smad5+/−, and 6 Smad5−/−embryos and are expressed as means ± SEMs. Results of replating assay were obtained from 36 wild-type, 36 heterozygous, and 72 Smad5−/− HPP-CFC macroscopic colonies of 3 yolk sac cultures, respectively, and are expressed as means ± SEMs. *represents data found to be significantly different from the corresponding control values (P < .05).

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