Investigation of embryonic globin gene expression by RT-PCR within day 3.5 and 4.0 EBs.

The arrows on the top of each photo represent the 10-fold dilution of samples. Hematopoietic-related genes were defined in collected day 3.5 EBs (A) and day 4 EBs (B). +/+T and −/−T in panel B denote samples in which additional TGF-β1 (2 ng/mL) was supplemented to wild-type and mutant EB-forming cultures.