Growth factors and growth factor receptors in B cells and PPC.

(A) Radioactive RT-PCR for gas6 in EBV-1 (lane 1), EBV-5 (lane 2), EBV-16 (lane 3), XG-1 (lane 4), XG-6 (lane 5), XG-16 (lane 6), MM1 (lane 7), MM2 (lane 8), MM3 (lane 9), RP (lane 10), and PPC1 to PPC3 (respectively, lanes 11 to 13). (B) Purified CD19⁺ PB B cells were single-stained with PE-conjugated anti-CD126 mAb (left). Dotted line indicates isotypic control. By comparison, cells obtained on day 6 of culture were stained with Cy chrome–labeled anti-CD20, FITC-labeled anti-CD38, and PE-labeled anti-CD126 mAb (right). Dotted line indicates isotypic control; bold line, CD20⁻/CD38⁺⁺ cells; solid line, CD20⁺/CD38^+/− cells.