Fig. 7.
Fig. 7. Expression of homing molecules in B cells and PPCs. / (A) Purified PB B cells (left panel) were stained with a PE-conjugated anti-CD162, anti-CCR2, or anti-CXCR5 mAb. PPCs were generated with the 6-day culture process described above and were stained with Cy chrome–labeled anti-CD20, FITC-labeled anti-CD38 and PE-conjugated anti-CD162, anti-CCR2, or anti-CXCR5 mAb. The right panel shows the expression of the homing molecules on gated CD20−/CD38++ cells. (B) Cell migration of B cells, PPCs, and tumor plasma cells. Purified B cells, XG-6 myeloma cell line, and sorted CD20−/CD38++ PPCs were seeded in the upper chamber of a Transwell with SDF-1 or MIP-3β in the lower chamber. Results are expressed as the percentage of input cells that migrated to the lower chamber.

Expression of homing molecules in B cells and PPCs.

(A) Purified PB B cells (left panel) were stained with a PE-conjugated anti-CD162, anti-CCR2, or anti-CXCR5 mAb. PPCs were generated with the 6-day culture process described above and were stained with Cy chrome–labeled anti-CD20, FITC-labeled anti-CD38 and PE-conjugated anti-CD162, anti-CCR2, or anti-CXCR5 mAb. The right panel shows the expression of the homing molecules on gated CD20−/CD38++ cells. (B) Cell migration of B cells, PPCs, and tumor plasma cells. Purified B cells, XG-6 myeloma cell line, and sorted CD20−/CD38++ PPCs were seeded in the upper chamber of a Transwell with SDF-1 or MIP-3β in the lower chamber. Results are expressed as the percentage of input cells that migrated to the lower chamber.

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