Regulation of STAT5 target genes during eosinophil differentiation.

(A) Protein lysates were prepared from CD34⁺ cells differentiated toward eosinophils for 3, 7, 10, 14, and 17 days. Western blot analysis was performed with antibodies against Bcl-2 (top panel) and p21^WAF/Cip1 (middle panel) and as a control for equal loading with an antibody against β-actin (bottom panel). The experiment shown is representative of 3 independent experiments. (B) CD34⁺ cells were transduced with eGFP, STAT5a, or STAT5aΔ750. After 7, 14, and 17 days of differentiation, eGFP-positive cells were sorted, and protein lysates were prepared and analyzed by Western blotting.