Fig. 1.
Fig. 1. Immunostaining of RBC membrane proteins from 4.2(-)Nancy and 4.2(-)Lisboa HS erythrocytes. / RhD-positive (DCCee), RhD-negative (dccEe), and Rhnull(amorph type) membrane proteins were used as controls. Anti–protein 4.2 polyclonal antibody was used to confim the lack of the 72-kDa band in the 2 samples of 4.2(-). Antibodies against the major members of the Rh complex were as follows: anti-Rh (D+ CcEe): PAb MPC8; anti-RhD: MoAb LOR15-C9; anti-RhAG: MoAb LA18.18; anti-CD47: MoAb 6H9. PAbs against the p55 and 4.1 proteins, which belong to the GPC-4.1-p55 membrane complex, were used as controls to show that similar amounts of membrane proteins were present on each lane.

Immunostaining of RBC membrane proteins from 4.2(-)Nancy and 4.2(-)Lisboa HS erythrocytes.

RhD-positive (DCCee), RhD-negative (dccEe), and Rhnull(amorph type) membrane proteins were used as controls. Anti–protein 4.2 polyclonal antibody was used to confim the lack of the 72-kDa band in the 2 samples of 4.2(-). Antibodies against the major members of the Rh complex were as follows: anti-Rh (D+ CcEe): PAb MPC8; anti-RhD: MoAb LOR15-C9; anti-RhAG: MoAb LA18.18; anti-CD47: MoAb 6H9. PAbs against the p55 and 4.1 proteins, which belong to the GPC-4.1-p55 membrane complex, were used as controls to show that similar amounts of membrane proteins were present on each lane.

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