Western blot analysis of the Rh complex proteins in CD47^−/− and 4.2^−/− gene-targeted mice.

Murine Rh and RhAG proteins (mRh and mRhAG) were detected by rabbit polyclonal antibodies raised against the C-terminal regions of the human Rh polypeptides) and of the murine RhAG glycoprotein, respectively. Murine CD47 (mCD47) was detected by the miap301 MAb raised against murine CD47. Normal expression of protein 4.2 in CD47^−/− mice was deduced after Coomassie or red Ponceau staining of the membrane proteins.