Similar secondary repopulation kinetics of clonally derived HSCs.

Each clone is indicated by the same color and number-letter combination as in Figure 1. Seven to 8 months after injection, BMCs from the indicated primary recipients were injected into 2 to 4 secondary hosts. Shown are the percentages of donor-type cells at each time point. (A) Control mice BM1 and BM2 received HSCs from primary hosts that originally were transplanted with multiclonal HSC grafts from freshly explanted BM. (B) Control mice bulk-1 and bulk-2 received HSCs from 2 primary hosts that originally were injected with populations of BMCs cultured on line S17. Each bulk primary host was derived from an independent experiment. (C,D) Daughter HSCs obtained from clonally engrafted primary hosts.