Modulation of chemokine gene expression following T-cell activation.

(A) Human CD4⁺ and CD8⁺ T lymphocytes were unstimulated or stimulated for 6 hours or 24 hours PMA, PMA+Iono, soluble anti–(α-)CD3 mAb plus PMA, or α-CD3 plus soluble α-CD28 mAb plus PMA, as indicated. Total RNA was prepared using Trizol, quantitated using OD₂₆₀, and analyzed by RT-PCR using IP-10, Ltn, MIP1α, MIP1β, and β-actin–specific primers, as outlined in “Materials and methods.” (B) IP-10, Ltn, MIP1α, and MIP1β bands were quantitated and values normalized to β-actin mRNA levels using ImageQuant software and are graphically represented. Shown are mean values ± SEMs of 2 experiments performed using isolated CD4⁺ and CD8⁺ T lymphocytes from different human donors.