Fig. 3.
Fig. 3. Calcineurin- and activation-dependent regulation of Ltn mRNA in human peripheral blood T cells. / Ltn mRNA levels in human PBLs were assessed by RPA. Cells were treated for 2 and 8 hours with ethanol diluent, anti-CD3 mAb (α-CD3) plus PMA, anti-CD28 mAb (α-CD28) plus PMA, anti-CD3 and ant-CD28 mAb (αCD3/28) plus PMA, PMA alone, or PMA+Iono, in the absence or presence of CsA as indicated. Total RNA was prepared (Figure 1) and RPA carried out as outlined (“Materials and methods”). (A) Results of 1 of 3 separate experiments, each using cells from different donors, are shown. (B) Ltn bands were quantitated and values were normalized to L32 mRNA levels using ImageQuant software; mean values ± SEMs of 3 experiments are graphically represented. Differences between Ltn mRNA following PMA+Iono stimulation were significantly different (P < .01) from baseline at 2 and 8 hours.

Calcineurin- and activation-dependent regulation of Ltn mRNA in human peripheral blood T cells.

Ltn mRNA levels in human PBLs were assessed by RPA. Cells were treated for 2 and 8 hours with ethanol diluent, anti-CD3 mAb (α-CD3) plus PMA, anti-CD28 mAb (α-CD28) plus PMA, anti-CD3 and ant-CD28 mAb (αCD3/28) plus PMA, PMA alone, or PMA+Iono, in the absence or presence of CsA as indicated. Total RNA was prepared (Figure 1) and RPA carried out as outlined (“Materials and methods”). (A) Results of 1 of 3 separate experiments, each using cells from different donors, are shown. (B) Ltn bands were quantitated and values were normalized to L32 mRNA levels using ImageQuant software; mean values ± SEMs of 3 experiments are graphically represented. Differences between Ltn mRNA following PMA+Iono stimulation were significantly different (P < .01) from baseline at 2 and 8 hours.

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