Calcineurin- and activation-dependent regulation of MIP-1β and IL-2 mRNA.

MIP-1β (A-B) and IL-2 (B) mRNA levels were assessed by RPA in human PBLs. (A) Cells were treated for 2 and 8 hours with ethanol diluent, α–CD3+PMA, α–CD28+PMA, α–CD3/28+PMA, PMA, or PMA+Iono (Figure2) in the absence or presence of CsA as indicated. (B) Cells were treated for 8 hours with ethanol diluent, α–CD3/28+PMA, or PMA+Iono in the absence or presence of CsA as indicated. Results of 1 of 3 separate experiments, each using cells from different donors, are shown.