Regulation of IP-10 mRNA by CsA.

IP-10 mRNA levels were assessed by RPA in human PBLs. (A) Cells were treated for 4, 8, and 24 hours with ethanol diluent, PMA+Iono, anti-CD3, or anti-CD28 mAb (α-CD3/28) plus PMA, in the absence or presence of CsA as indicated. (B) Cells were treated for 8 hours with ethanol diluent, α–CD3+PMA, α–CD28+PMA, α–CD3/28+PMA, PMA, or PMA+Iono in the absence or presence of CsA as indicated. RPA was carried out as outlined in “Materials and methods.” IP-10 bands were quantitated and values normalized to L32 mRNA levels using ImageQuant software and are graphically represented. Results of 1 of 3 representative experiments, performed using human PBLs from different human donors, are shown.